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PEN treatment normalizes serum protein markers of cardiac injury (CK-MB, LDH, Cardiac troponin I) assessed by ELISA. Serum levels of cardiac markers were significantly increased by DOX, while PEN administration normalized them. DOX: Doxorubicin; PEN: Penicillamine; LDH: Lactic acid dehydrogenase; CK-MB: Creatine kinase-MB. *** p < 0.001, **** p < 0.0001, ns = non-significant.

Journal: Biomolecules

Article Title: Copper Chelation by Penicillamine Protects Against Doxorubicin-Induced Cardiomyopathy by Suppressing FDX1-Mediated Cuproptosis

doi: 10.3390/biom15091320

Figure Lengend Snippet: PEN treatment normalizes serum protein markers of cardiac injury (CK-MB, LDH, Cardiac troponin I) assessed by ELISA. Serum levels of cardiac markers were significantly increased by DOX, while PEN administration normalized them. DOX: Doxorubicin; PEN: Penicillamine; LDH: Lactic acid dehydrogenase; CK-MB: Creatine kinase-MB. *** p < 0.001, **** p < 0.0001, ns = non-significant.

Article Snippet: The serum levels of lactic dehydrogenase (LDH), creatine kinase MB (CK-MB), and cardiac troponin I (CTnI) were measured using commercially available kits: LDH assay endpoint kit (MG; Egypt, cat. no. MG283001 ), CK-MB ELISA kit (Elabscience, Houston, TX, USA, CAT: E-CL-R0722), and CTnI ELISA kit (Elabscience, Houston, TX, USA, CAT: E-EL-R1253).

Techniques: Enzyme-linked Immunosorbent Assay

Cardiac tissue contents of oxidative stress markers (glutathione peroxidase, malondialdyde and superoxide dismutase) in DOX and PEN-treated rats measured by ELISA. The DOX treatment induced an increase in MDA level with depletion of both SOD and GPx. PEN administration reversed these changes. DOX: doxorubicin; PEN: penicillamine; MDA: malondialdehyde; SOD: superoxide dismutase, GPx: glutathione peroxidase. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = non-significant.

Journal: Biomolecules

Article Title: Copper Chelation by Penicillamine Protects Against Doxorubicin-Induced Cardiomyopathy by Suppressing FDX1-Mediated Cuproptosis

doi: 10.3390/biom15091320

Figure Lengend Snippet: Cardiac tissue contents of oxidative stress markers (glutathione peroxidase, malondialdyde and superoxide dismutase) in DOX and PEN-treated rats measured by ELISA. The DOX treatment induced an increase in MDA level with depletion of both SOD and GPx. PEN administration reversed these changes. DOX: doxorubicin; PEN: penicillamine; MDA: malondialdehyde; SOD: superoxide dismutase, GPx: glutathione peroxidase. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = non-significant.

Article Snippet: The serum levels of lactic dehydrogenase (LDH), creatine kinase MB (CK-MB), and cardiac troponin I (CTnI) were measured using commercially available kits: LDH assay endpoint kit (MG; Egypt, cat. no. MG283001 ), CK-MB ELISA kit (Elabscience, Houston, TX, USA, CAT: E-CL-R0722), and CTnI ELISA kit (Elabscience, Houston, TX, USA, CAT: E-EL-R1253).

Techniques: Enzyme-linked Immunosorbent Assay